The principal goal of the NBSIS 2017 is to create a forum for contributed and invited papers on nano-bio sensing, imaging, and spectroscopy and other related topics covering not only basic research but also hardware developments and system applications. The proposed technical program includes sessions on the following topics:     Emerging microscopic techniques Spectroscopy techniques including Raman Super-resolution microscopy Near-field detection techniques including NSOM Confocal imaging techniques Optical fiber sensors Multiphoton imaging Resonator-based detection techniques including nano/microcavities and whispering gallery modes Photoacoustic imaging and microscopy Sensors based on nanophotonics and plasmonics Optical coherence tomography I Molecular detection methods Optical coherence tomography II Sensors using novel optical materials such as photonic crystals and metamaterials In vivo imaging for clinical applications Optofluidics Optics in neuroscience Terahertz techniques